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ZORBAX 80 Ectend-C18
A ƙarƙashin yanayin high PH (PH = 11.5), ingancin ginshiki yana da tsawon rayuwa; Musamman biyu-layout binding da biyu rufe karshen samar da high PH k
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ZORBAX 80ÅEctend-C18ginshiƙi
A cikin yanayin high PH (PH = 11.5), ingancin ginshiki yana da tsawon rayuwa. Musamman biyu-layout binding da biyu rufe karshen samar da high PH kwanciyar hankali; Yana da mafi girma column tasiri da kuma mafi kyau peak siffar fiye da polymer tushen chromatography ginshike; Inganta kiyayewa, rabuwa da kuma peak siffar alkaline mahada; LC / MS raba da babban hankali ga polypeptides.
Agilent ZORBAX Extend-C18 Chromatography Column yana amfani da sabbin fasahohin haɗuwa na C18-C18 mai haɗuwa biyu, wanda ke ba da damar haɓaka rabuwa mai girma ta amfani da silicone-tushen chromatography column a cikin yanayin high PH. A ƙarƙashin yanayin high PH, mahaɗan alkali da ba a caji ba zai yi hulda da silicone da ke ƙasa ba. An sami ingantaccen rabuwa tare da kyakkyawan ƙwarewar ƙwarewa, kuma an inganta rabuwa. Babban rabuwa da PH yana da kyakkyawan zaɓi don rabuwa da mahaɗan da ke da ƙarfin kwanciyar hankali ko sauƙin narkewa a cikin mafita mai ƙarfin PH. Fluid buffers don high PH yanayi sun hada da triethylamine, pyrrolide, amino acetic acid, borate da ammonium hydroxide. Ammonium hydroxide na PH 10.5 shine kyakkyawan mai canzawa na tafiya don polypeptides da ƙananan kwayoyin LC / MS tare da mafi girman hankali idan aka kwatanta da trifluoroacetic acid wanda ke ƙunshe da tafiya a ƙananan yanayin PH. Extend-C18 Chromatography column yana da kwanciyar hankali a cikin kewayon PH 2-11.5 kuma yana da kyakkyawan ƙwarewa ga duk nau'ikan mahada. Extend-C18 Chromatography column yana ba da ƙarin zaɓi a ƙarƙashin yanayin PH.
III. Chromatography column aiki nuna alama
Binding mataki |
Diameter na |
Compare surface yankin |
iyakar zafin jiki * |
PH kewayon |
Ƙarshe |
Karbon kaya |
ZORBAX Extend-C18 |
80Å |
180m2/g |
60℃ |
2.0-11.5 |
biyu |
12.5% |
* A kasa da PH 8, iyakar zafin jiki ita ce 60 ° C, a PH 8-11.5 ita ce 40 ° C.
4. Agilent ZORBAX Extend-C18 Chromatography column daban-daban bayanan bayani:
Alamar |
Bayani |
Bayani (mm) |
Girman ƙwayoyi (µm) |
Lambar sassa |
Standard ginshike (babu bukatar katin sa, 400bar) | ||||
|
Ginshiƙin bincike |
4.6＊250 |
5 |
770450-902 |
|
Ginshiƙin bincike |
4.6＊150 |
5 |
773450-902 |
|
Ginshiƙin bincike |
4.6＊50 |
5 |
746450-902 |
|
Rapid raba ginshike |
4.6＊150 |
3.5 |
763953-902 |
|
Rapid raba ginshike |
4.6＊100 |
3.5 |
764953-902 |
|
Rapid raba ginshike |
4.6＊75 |
3.5 |
766953-902 |
|
Rapid raba ginshike |
4.6＊50 |
3.5 |
735953-902 |
|
Rapid raba HT ginshike, 600bar |
4.6＊100 |
1.8 |
728975-902 |
|
Rapid raba HT ginshike, 600bar |
4.6＊50 |
1.8 |
727975-902 |
|
Rapid raba high gudu ginshike, 400bar |
4.6＊50 |
1.8 |
722975-902 |
|
Rapid raba HT ginshike, 600bar |
4.6＊30 |
1.8 |
724975-902 |
|
Rapid raba HT ginshike, 600bar |
4.6＊20 |
1.8 |
726975-902 |
|
Mai narkewa Ajiye Column |
3.0＊250 |
5 |
770450-302 |
|
Mai narkewa Ajiye Column |
3.0＊150 |
5 |
773450-302 |
|
Inganta mai narkewa ceton ginshike |
3.0＊150 |
3.5 |
763954-302 |
|
Inganta mai narkewa ceton ginshike |
3.0＊100 |
3.5 |
764953-302 |
|
Inganta mai narkewa ceton ginshike |
3.0＊50 |
3.5 |
735954-302 |
|
Mai narkewa ceton HT ginshike, 600bar |
3.0＊100 |
1.8 |
728975-302 |
|
Mai narkewa ceton HT ginshike, 600bar |
3.0＊50 |
1.8 |
727975-302 |
|
Mai narkewa ceton HT ginshike, 600bar |
3.0＊30 |
1.8 |
724975-302 |
|
Mai narkewa ceton HT ginshike, 600bar |
3.0＊20 |
1.8 |
726975-302 |
|
Ƙananan Diameter Column |
2.1＊150 |
5 |
773700-902 |
|
Ƙananan Diameter Column |
2.1＊50 |
5 |
760450-902 |
|
Rapid raba m diameter ginshiƙi * |
2.1＊100 |
3.5 |
761753-902 |
|
Rapid raba m diameter ginshiƙi * |
2.1＊50 |
3.5 |
735700-902 |
|
Ƙananan diamita RRHT ginshiƙi, 600bar |
2.1＊100 |
1.8 |
728700-902 |
|
Ƙananan diamita RRHT ginshiƙi, 600bar |
2.1＊50 |
1.8 |
727700-902 |
|
Ƙananan diamita RRHT ginshiƙi, 600bar |
2.1＊30 |
1.8 |
724700-902 |
|
Ƙananan diamita RRHT ginshiƙi, 600bar |
2.1＊20 |
1.8 |
726700-902 |
|
Rapid raba microdiameter ginshike * |
1.0＊150 |
3.5 |
763600-902 |
|
Rapid raba microdiameter ginshike * |
1.0＊50 |
3.5 |
765600-902 |
|
Rapid raba microdiameter ginshike * |
1.0＊30 |
3.5 |
761600-902 |
|
Microdiameter tsabar kariya tsabar core, 3 / kunshin |
1.0＊17 |
5 |
5185-5923 |
|
Kariya pillar, 4 / kunshin |
4.6＊12.5 |
5 |
820950-930 |
|
Kariya pillar, 4 / kunshin |
2.1＊12.5 |
5 |
821125-930 |
|
Kare Column Set |
|
|
820888-901 |
PrepHT katin gyare-gyare (yana buƙatar gyare-gyare mai haɗi, 820400-901) | ||||
|
PrepHT katin ginshike |
21.2＊150 |
5 |
770150-902 |
|
PrepHT |
21.2＊100 |
5 |
770100-902 |
|
PrepHT |
21.2＊50 |
5 |
770050-902 |
|
PrepHT madaidaiciya, 2 / kunshin |
|
|
820400-901 |
|
PrepHT kariya pillar, 2 / kunshin |
17＊7.5 |
5 |
820212-930 |
|
Kariya pillar katin soket |
|
|
820444-901 |
* RR: Fast raba ginshike 3.5μm
** RRHT: sauri raba HT ginshike 1.8μm
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