FluorCam rufe chlorophyll fluorescence imaging tsarin

PSIBabban masanin kimiyya na kamfanin, Farfesa Nedbal da Shugaban kamfanin, Dr. Trtilek da sauransu, sun fara haɗuwa da fasahar fluorescence ta PAM tare da fasahar CCD don haɓaka da samar da tsarin hoton fluorescence na FluorCam a duniya a 1996 (Heck et al., 1999; Nedbal da sauransu, 2000; Govindjee and Nedbal, 2000）。 FluorCam chlorophyll fluorescence imaging fasaha ya zama wani muhimmin ci gaba a cikin 1990's chlorophyll fluorescence fasaha, sa masana kimiyya a kan photosynthesis da binciken chlorophyll fluorescence nan da nan shiga 2D duniya da kuma microscopic duniya. A halin yanzu, kamfanin PSI ya zama mafi iko, mafi yawan amfani, mafi cikakken nau'i, mafi yawan takardun da aka buga na chlorophyll fluorescence imaging masana'antun masana'antun duniya

Hoton sama da hagu shine fasahar daukar hoto ta FluorCam da Nedbal da sauransu suka tsara a shekarun 1990 (Binciken Photosynthesis, 66: 3-12, 2000) da dama shine hoton launi na lemon da hoton daukar hoto ta chlorosynthesis (Photosynthetica, 38: 571-579, 2000)

FluorCamRufe-irin Chlorophyll fluorescence imaging tsarin ne sosai hadedde, sosai m, sauki don amfani, aikace-aikace mai fadi na high karshen Chlorophyll fluorescence fasaha kayan aiki, high hankali CCD ruwan tabarau, 4 m LED haske tushen allon da kuma sarrafa tsarin da dai sauransu hadedde a cikin wani duhu daidaitawa aiki akwati, shuka samfurin sanya a kan allon a cikin duhu daidaitawa aiki akwati, allon mataki 7 tsayi daidaitawa; Haske tushen samar da wutar lantarki ta hanyar high kwanciyar hankali samar da rukuni, 4 high makamashi, high kwanciyar hankali LED haske tushen allon uniformly haskaka a kan shuka samfurin, imaging yankin iya zuwa 13×13 cmTsarin sarrafawa yana haɗe da kwamfuta ta hanyar USB kuma yana sarrafawa da tattara bayanan bincike ta hanyar shirye-shiryen FluorCam software. Ana amfani da shi don wasu tsire-tsire kamar ganye na tsire-tsire da 'ya'yan itace, dukan tsire-tsire ko tsire-tsire da yawa da aka noma, tsire-tsire da ƙananan tsire-tsire, algae da sauransu, ana amfani da shi sosai a cikin tsire-tsire ciki har da algae photosynthesis physiological muhalli, tsire-tsire matsala tilasta physiology da sauƙi, porous aiki, tsire-tsire muhalli kamar ƙasa mai nauyi karfe gurɓataccen amsa da kuma binciken halitta, tsire-tsire juriya ganowa da kuma binciken, amfani

Main fasali:

· Tsarin da aka haɗa a cikin akwatin aiki na duhu mai daidaitawa, mai sauƙin aiki da sauƙin motsi, ana iya yin nazarin auna hoton duhu mai daidaitawa a cikin dakin gwaje-gwaje ko waje

·High hankali CCD ruwan tabarau, lokaci ƙuduri har zuwa 50 hotuna a kowane dakika, sauri kama chlorophyll fluorescent transient, hoto yankin har zuwa 13x13cm

· Yana da kawai duniya iya gudanar da OJIP sauri fluorescence dynamic imaging nazarin high-karshen chlorophyll fluorescence fasahar kayan aiki, iya samun OJIP sauri chlorophyll fluorescence dynamic curve da Mo (OJIP curve farko inclination), OJIP m yanki, Sm (ma'auni na makamashi da ake bukata don rufe duk haske amsa cibiyoyin), QY, PI (Performance Index) da sauransu 26 sigogi

· ne kawai high-karshen chlorophyll fluorescence fasahar kayan aiki a duniya iya gudanar da QA re-oxidation dynamic imaging nazarin, iya gudanar da daya-juyawa cike haske flash (STF) chlorophyll fluorescence induced dynamics, haske karfi a100µs120,000 μmol (photons) / m².s a cikin

· Tare da mafi aiki, editable Chlorophyll Fluorescence gwaji shirye-shirye (Protocols), ciki har da snapshot yanayin, Fv / Fm, Kautsky induced tasiri, 2 Chlorophyll Fluorescence kashewa nazarin (NPQ) protocols (2 saiti na musamman ga haske tsare-tsare), LC haske amsa curve, PAR sha da NDVI hoto nazarin, QA re-oxidation dynamics nazarin, OJIP sauri fluorescence dynamics nazarin da GFP kore fluorescent furotin hoto da sauransu

· Za a iya gudanar da atomatik maimaita image ma'auni nazarin, tsoho gwaji tsari (Protocols), yawan ma'auni da kuma tsawon lokaci, tsarin zai ta atomatik zagaye gudanar da image ma'auni, da kuma ta atomatik ajiye bayanai a kwamfuta da lokaci kwanan wata (tare da lokaci hatimi); Har ila yau, biyu gwaji shirye-shirye (Protocols) za a iya tsara; Misali don yin tsarin ta atomatik gudanar da Fv / Fm a rana, ta atomatik gudanar da nazarin NPQ da sauransu da dare

·Tare da launi biyu photochemical haske motsawa haske tushen, misali daidaitawa don ja da fari, zaɓi da ja da shuɗi da sauran biyu-band photochemical haske, biyu launi photochemical haske za a iya amfani da shi tare da daban-daban rabo, don gwaji daban-daban haske inganci a kan amfanin gona / shuka

· Zaɓi tare da TetraCam launi imaging module, max hoto yankin 20 × 25cm don ganye ko shuka siffar imaging bincike da kuma chlorophyll fluorescence imaging bambanci bincike

fasaha sigogi:

· Ma'aunin haske shine 617nm mai daidaitawa ja haske, wanda ya ƙunshi 2 arrays 144 LEDs tare da tsawon 10μs - 100μs daidaitawa;

· Bi launi photochrome haske, misali tare da 2 ja haske + 2 farin haske, zaɓi tare da 2 ja haske + 2 blue haske ko wasu wavelength haske tushen haɗuwa, Actinic1 haske mai ƙarfi300µmol(photons)/m².s， Actinic2光强2000μmol (photons) / m².s; Max photochemical haske za a iya haɓaka zuwa 3000μmol (photons) / m².s. Bi-launi photochemical haske za a iya amfani da shi tare da daban-daban rabo don gwaji daban-daban haske siffofin a kan amfanin gona / shuka

· Hasken haske mai ƙarfi zai iya zuwa 4000μmol (photons) / m².s, wanda za a iya haɓaka zuwa 6000 μmol (photons) / m².s,QAReoxidation bincike guda juyawa saturated flash STF har zuwa 120000μmol (photons) / m².s

· Photochemical haske da cike haske flash ya kunshi 2 nau'ikan high karfi high kwanciyar hankali LED haske tushen allon, kowane haske tushen allon ya kunshi 8 × 9 LEDs array, kuma yana da wani saman biyu launi haske tushen (740nm infrared haske tushen da 660nm ja haske tushen) don PAR sha da NDVI imaging ma'auni; High karfi High kwanciyar hankali LED samar da ci gaba, kwanciyar hankali, daidai haske tushen, ba saboda da yawan haske karfi da rauni LEDs (misali daruruwan) haifar da haske tushen rashin kwanciyar hankali, gajeren rayuwa da sauran matsaloli (amfani da yawan rauni haske LED don biyan da rashin isasshen kowane LED, zai haifar da tsarin kuskure yawan inganta, duk wani LED matsala zai haifar da tsarin rashin kwanciyar hankali ko ma ba za a iya amfani da)

· auna sigogi: Fo, Fo’, Fs, Fm, Fm’, Fp, FtDn, FtLn, Fv, Fv'/ Fm'，Fv/ Fm ,Fv',Ft,ΦPSII, NPQ_Dn, NPQ_Ln, Qp_Dn, Qp_Ln, qN, qP，QY, QY_Ln, Rfd, ETR Fiye da 50 chlorophyll fluorescence sigogi da kuma PAR sha da NDVI shuka spectrum reflective index, kowane sigogi zai iya nuna 2D fluorescence launi hoto

· Tare da cikakken atomatik auna shirye-shirye (protocol), kyauta gyara atomatik auna shirye-shirye

a) Fv/Fm: auna sigogi sun hada da Fo, Fm, Fv, QY da sauransu

b) KautskyTasirin induction: Fo, Fp, Fv, Ft_Lss, QY, Rfd da sauran sigogin fluorescence

c) Fluorescent kashewa bincike: Fo, Fm, Fp, Fs, Fv, QY, Φ_II， Fiye da 50 sigogi NPQ, Qp, Rfd, QL da sauransu

d) Light amsa curve LC: Fo, Fm, QY, QY_Ln, ETR da sauransu fluorescent sigogi

e) PARsha tare da NDVI (zaɓi)

f) QAReoxidation Dynamics (zaɓi)

g) GFPStatic fluorescence imaging ma'auni (zaɓi)

h) OJIPNazarin Fluorescent Dynamics mai sauri (zaɓi): Mo (ƙarancin farko na OJIP curve), OJIP Fixed Area, Sm (ma'auni na makamashi da ake buƙata don rufe duk cibiyoyin amsawa na haske), QY, PI da sauransu 26 sigogi

• Babban SensitivityTOMI-1CCDfirikwensin:

a) Hoton ƙuduri: 720 × 560 pixels

b) Lokaci ƙuduri: 50 frames a kowane dakika

c) A/DSauya ƙuduri: 12bit (4096 launin toka)

d) Abubuwan girma: 8.6μm × 8.3μm

e) Sadarwa Mode: Gigabit Ethernet

§ High ƙuduri TOMI-2 CCD firikwensin (zaɓi)

a) Binciken CCD

b) Matsakaicin ƙudurin hoto: 1360 × 1024 pixels

c) Lokaci ƙuduri: har zuwa 20 frames a kowane dakika a kan mafi girman hoto ƙuduri

d) A/DSauya ƙuduri: 16-bit (65,536 launin toka)

e) Abubuwan girma: 6.45μm × 6.45μm

f) Gudanar da yanayin: 1) Dynamic video yanayin don ma'auni na chlorophyll fluorescence sigogi; 2) Snapshot yanayin don auna fluorescent furotin da fluorescent launi kamar GFP

g) Sadarwa Mode: Gigabit Ethernet

· Hoton yanki: 13×13cmZa a iya yin nazarin hoto na ganyen shuka, ƙwayoyin shuka, algae, moss, ƙasa, dukan shuka ko shuka da yawa, 96 rami, 384 rami, da dai sauransu

· 7Filtration Wave & Chlorophyll fluorescence tace, PAR sha da NDVI imaging ma'auni tace, za a iya zaɓar da sauran tace kamar yadda ake bukata (zaɓi)

· QABinciken hoto na redox (zaɓi): Ana iya auna STF fluorescence dynamics analysis tare da hasken haske mai ƙarfi har zuwa 120000 μmol (photons) / m².s a cikin 100μs

· OJIPRapid Fluorescent Dynamics Module (Zaɓi): Lokaci ƙuduri har zuwa 1µs, ƙididdigar ƙididdigar OJIP curve tare da fiye da ashirin sigogi masu dacewa sun haɗa da: Fo, Fj, Fi, P ko Fm, Vj, Vi, Mo, Yankin, Yankin gyara, Sm, Ss, N (QA rage yawan juyawa), Phi--_Po, Psi_o, Phi_Eo, Phi_Do, Phi_pav, ABS / RC (shafin haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na haske na has PI_ABS("Performance" index ko Survival index bisa sha haske quantum kwarara), PIcs ("Performance" index ko Survival index bisa sashe)

FluorCamChlorophyll fluorescence hoton bincike software aiki: tare da Live (live gwaji), Protocols (gwaji shirin zaɓi musamman), Pre-processing (hoton pre-processing), Sakamakon (hoton bincike sakamakon) da sauransu aiki menu

· Abokin ciniki musamman gwaji shirye-shirye yarjejeniya (protocols): Za a iya saita lokaci (kamar auna haske tsawon lokaci, photochemical haske tsawon lokaci, auna lokaci, da dai sauransu), haske karfi (kamar daban-daban haske ingancin photochemical haske karfi, saturated haske karfi, modulation auna haske, da dai sauransu), tare da keɓaɓɓun gwaji shirye-shirye harshe da rubutun, masu amfani kuma iya samun kyauta don ƙirƙirar sabon gwaji shirye-shirye ta amfani da wizard shir

· Auto auna analytical aiki: Za a iya saita wani gwaji shirin (Protocol) ta atomatik unmanned zagaye image auna, maimaitawa da kuma tsakanin lokaci abokin ciniki musamman, image auna bayanai ta atomatik ajiye a kwamfuta ta hanyar lokaci kwanan wata (tare da lokaci hatimi)

· Snapshot yanayin: Ta hanyar snapshot hoto yanayin, za a iya kyauta daidaita haske ƙarfi, shutter lokaci da kuma hankali samun m shuka samfurin steady halin fluorescence da nan take fluorescence hotuna

· Pre-imaging: Software na shirin zai iya gano samfuran tsire-tsire da yawa ko yankuna da yawa ta atomatik, kuma zai iya zaɓar yankin sha'awa (ROI) da hannu. Cikakken zaɓin hannu na iya zama murabba'i, zagaye, wani polygon ko ɓangare. Software auna ta atomatik bincike fluorescence dynamics curves da daidai sigogi na kowane samfurin da kuma zaɓi yanki, samfurin ko yanki adadin Unlimited (> 1000)

· Bayanan bincike yanayin: tare da "siginar lissafi recalculation" yanayin (arithmetic matsakaicin) da kuma "siginar matsakaicin recalculation" yanayin, zaɓi "siginar lissafi recalculation" yanayin a cikin yanayin high siginar amo rabo, zaɓi "siginar matsakaicin recalculation" yanayin a cikin yanayin low siginar amo rabo don tace kuskuren da amo ya kawo

· Output sakamakon: High lokaci ƙuduri fluorescence dynamic chart, fluorescence dynamic canji video, fluorescence sigogi Excel fayil, histogram, daban-daban sigogi hoto chart, daban-daban ROI fluorescence sigogi jerin da sauransu

· Dark daidaitawa aiki akwatin, gina-haske tushen, CCD ruwan tabarau, kore raƙuman ruwa da tace, sarrafawa na'ura, zafi na'ura, da dai sauransu, sauki duhu daidaitawa aiki, samfurin dandali 36x30cm, tsawo matakin 7 daidaitawa, samfurin (dukan shuka) max tsawo har 12cm

· Bayar da Optical nau'i: tsayayye ko m (sinus nau'i)

· Spectrum amsa:540 nmMafi inganci na quantum (70%) a 400 nm da 650 nm

· Karanta amo:Kasa da 12eRMS, yau da kullun 10e

· Cikakken tarko iya:fiye da 70,000 e (unbinned)

· Bios：Firmware da za a iya haɓaka

· Sadarwa:Gigabit Ethernet

· Girma:471 mm(W)×473 mm (D)×512 mm (H)

· nauyi:Appr. 40 kg

· Power shigarwa:Appr. 1100 W

· Wutar lantarki:90–240 V

Saitawa maki:

1. Host tsarin, ciki har da duhu daidaitawa aiki akwatin, gina-in LEDs haske tushen, kore Wave & tace, CCD ruwan tabarau, sarrafawa na'ura, high daidaitawa samfurin partitions, zafi na'ura da sauransu

2. High kwanciyar hankali Power Converter

3. FluorCamTsarin sarrafawa da data analysis software

4. kwamfutar tafi-da-gidanka

Asalin:Czech PSI

da aka haɗa:Sauran tsarin daukar hoto na FluorCam

1. FluorCamChlorophyll fluorescence imaging tsarin da za a iya amfani da shi tare da LCProSD photosynthetic, Licor6400 photosynthetic da sauransu

2. FluorCamTsarin daukar hoto na Chlorophyll Fluorescent: Yankin hoto na 3.5x3.5cm tare da duhu mai dacewa da ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-ganyen ganye-gan

3. FluorCamTsarin daukar hoto na Chl / GFP fluorescence: Don ƙarin fasalin tsarin daukar hoto na fluorescence, ana iya yin nazarin hoto na chlorophyll fluorescence da nazarin hoto na GFP green fluorescence protein a lokaci guda

4. FluorCamRufe Chlorophyll fluorescence hoto tsarin: LED haske tushen, CCD fluorescence sa ido ruwan tabarau, kula da na'urori da dai sauransu hadedde a cikin duhu daidaitawa aiki akwatin samar da wani cikakken baƙi tsarin, shi ne kawai a duniya da za a iya gudanar da QA re-oxidation dynamics da kuma OJIP ma'auni bincike da Chlorophyll fluorescence hoto tsarin, hoto yankin 13x13cm

5. FluorCamRufe Chl / GFP fluorescence imaging tsarin: domin fadada sigar rufe chlorophyll fluorescence imaging tsarin, za a iya gudanar da lokaci guda chlorophyll fluorescence imaging analysis da GFP kore fluorescence furotin imaging analysis

6. FluorCamBudewa Chlorophyll fluorescence imaging tsarin: Modular tsarin, tare da high scalability, free zaɓi daban-daban motsawa haske tushen da kuma dacewa tace don yi image nazari na Chlorophyll fluorescence m da kuma m yanayin fluorescence da dai sauransu, ruwan tabarau tsayi daidaitacce, image yankin 13x13cm

7. FluorCamBude babban tsarin daukar hoto na chlorophyll fluorescence: Yankin hoto ya kai 20x20cm

8. FKMMulti-spectrum fluorescence dynamic microimaging da kuma spectrum analysis tsarin: Multi-excited haske, Multi-spectrum fluorescence imaging da kuma spectrum analysis, za a iya yi microimaging analysis da kuma spectrum analysis na chlorophyll fluorescence dynamics, QA re-oxidation, OJIP sauri fluorescence dynamics, kuma za a iya yi microimaging analysis na GFP fluorescence, cell fluorescence staining da dai sauransu

9. FluorcamTsarin daukar hoto na Chlorophyll Fluorescent: Babban dandalin daukar hoto na Chlorophyll Fluorescent ya shigar da shi a kan kayan aiki tare da ƙafa, yana da sauƙin motsawa, dandalin daukar hoto yana iya motsawa sama da ƙasa, yankin hoto har zuwa 35x35cm

10. FluorCamTsarin daukar hoto na samfurin samfurin: Babban dandalin daukar hoto zai iya daukar hoto na samfurin samfurin a kan 100-500cm, tsawon yankin daukar hoto na misali shine 400cm, dandalin daukar hoto zai iya daidaita daukar hoto na atomatik a fadin samfurin samfurin, zaɓi zai iya daukar hoton daukar hoto na RGB na gaskiya don cimma hoton daukar hoto na fluorescence da nazarin hoton launi na gaskiya

11. FluorCamMulti-spectrum fluorescence hoto tsarin: ne mai da yawa motsawa haske, Multi-spectrum fluorescence hoto tsarin, ba kawai za a iya yi hoto nazarin na chlorophyll fluorescence, har ma da UV UV haske motsawa F440 (blue fluorescence), F520 (kore fluorescence), F690 (ja fluorescence) da F740 (infrared fluorescence) don yin hoto nazarin don cikakken bincike gano shuka tilasta da juriya, tare da misali daidaitawa, fadada daidaitawa da manyan daidaitawa 3 samfuran

12. PlantScreenChlorophyll fluorescence da RGB ainihin launi atomatik bincike hoto tsarin: shi ne tushe version na PlantScreen jerin high-gudanarwa shuka phenotype hoto nazarin tsarin, zai iya yin hoto nazarin na Chlorophyll fluorescence da shuka RGB ainihin launi don nazarin gano aiki phenotypes da kuma morphological phenotypes na shuka, atomatik bincike kewayon 60x129cm, lokaci lokaci da kuma samun 4D (XYZ 3D wuri bayanai da kuma lokaci bayanai) ma'auni bayanai